PENTAGON ELECTROCONFORMATIONAL COUPLING

Research Rife Machine Technology – Pentagon Release
https://rifemachine.com/rife-machine-pentagon-release/
Previous work has shown that membrane ATPases can extract free energy from applied oscillating electric fields for doing chemical work, e.g. to synthesize ATP from ADP and P(i) or to transport Rb and Na ions against their respective electrochemical gradient. Data of these experiments are briefly reviewed. Electroconformational Coupling (ECC) is used to interpret these results. Computer analysis of a four state cyclic enzyme mechanism reproduces many experimental features. It is shown that a coulombic interaction between an enzyme and an alternating electric field (ac) can cause the enzyme to oscillate between different conformational states. If the frequency of the applied field matches the kinetic characteristics of the system and the amplitude matches the energy required for inducing productive catalytic cycling, a phenomenological resonance between catalytic reaction and the periodic field is generated. A condition necessary for achieving energy coupling is the kinetic bias arising from the binding energy of the ligand. Analysis indicates that only dynamic electric fields, i.e. oscillating or fluctuating fields, can propel the cyclic reaction of the enzyme catalysis, and thus be effective for transducing energy. A stationary transmembrane electric field must be modulated, e.g. by opening and closing of an ion channel, to become oscillatory in order to produce the same effect. We propose that ECC is a fundamental process of cellular energy and signal transductions. Here, many membrane associated events are reduced to Michaelis-Menten types of enzyme catalytic reactions and they are thus amenable to the quantitative analysis of chemical kinetics.

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